Application of affinity aqueous two-phase systems for the fractionation of CD133(+) stem cells from human umbilical cord blood.

@article{GonzlezGonzlez2015ApplicationOA,
  title={Application of affinity aqueous two-phase systems for the fractionation of CD133(+) stem cells from human umbilical cord blood.},
  author={Mirna Gonz{\'a}lez-Gonz{\'a}lez and Marco Rito-Palomares},
  journal={Journal of molecular recognition : JMR},
  year={2015},
  volume={28 3},
  pages={
          142-7
        }
}
In a further attempt to establish a novel stem cell primary recovery strategy, the use of aqueous two-phase systems (ATPS) complemented with the use of antibodies (known as immunoaffinity ATPS) is explored in this work. This type of liquid-liquid extraction systems exploits antigen-antibody affinity and represents a novel and selective approach for the purification of stem cells. The proposed bioengineering strategies include the implementation of traditional [polyethylene glycol (PEG), dextran… CONTINUE READING
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