Application of adaptive laboratory evolution to overcome a flux limitation in an Escherichia coli production strain.

@article{Tokuyama2018ApplicationOA,
  title={Application of adaptive laboratory evolution to overcome a flux limitation in an Escherichia coli production strain.},
  author={Kento Tokuyama and Yoshihiro Toya and Takaaki Horinouchi and Chikara Furusawa and Fumio Matsuda and Hiroshi Shimizu},
  journal={Biotechnology and bioengineering},
  year={2018},
  volume={115 6},
  pages={1542-1551}
}
Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of the succinate of the designed gene deletion strain was improved by… CONTINUE READING