Application of a ribosomal DNA integration vector in the construction of a brewer's yeast having alpha-acetolactate decarboxylase activity.

@article{Fujii1990ApplicationOA,
  title={Application of a ribosomal DNA integration vector in the construction of a brewer's yeast having alpha-acetolactate decarboxylase activity.},
  author={Toshihiko Fujii and Keiji Kondo and Fumio Shimizu and Hidetaka Sone and Junichi Tanaka and Takehiro Inoue},
  journal={Applied and environmental microbiology},
  year={1990},
  volume={56 4},
  pages={997-1003}
}
An integration plasmid, pIARL28, containing the ribosomal DNA gene as a homologous recombination sequence was constructed for introduction of the alpha-acetolactate decarboxylase gene into brewer's yeast. The transformation efficiency of pIARL28 was 20- to 50-fold higher than those of the other YIp vectors, as yeast cells had approximately 140 copies of the ribosomal DNA gene. All transformants showed very high alpha-acetolactate decarboxylase activity due to the multiple integrated copies of… CONTINUE READING

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