Application of a recombinase polymerase amplification (RPA) assay and pilot field testing for Giardia duodenalis at Lake Albert, Uganda

@article{MolinaGonzalez2020ApplicationOA,
  title={Application of a recombinase polymerase amplification (RPA) assay and pilot field testing for Giardia duodenalis at Lake Albert, Uganda},
  author={Sandra Molina-Gonzalez and Tapan Bhattacharyya and Hajri Al-shehri and Kate Poulton and S. Allen and M. Miles and Moses Arianitwe and E. Tukahebwa and B. Webster and J. Russell Stothard and A. Bustinduy},
  journal={Parasites & Vectors},
  year={2020},
  volume={13}
}
  • Sandra Molina-Gonzalez, Tapan Bhattacharyya, +8 authors A. Bustinduy
  • Published 2020
  • Biology, Medicine
  • Parasites & Vectors
  • Giardia duodenalis is a gastrointestinal protozoan causing 184 million cases of giardiasis worldwide annually. Detection is by microscopy or coproantigen assays, although sensitivity is often compromised by intermittent shedding of cysts or trophozoites, or operator expertise. Therefore, for enhanced surveillance field-applicable, point-of-care (POC), molecular assays are needed. Our aims were to: (i) optimise the recombinase polymerase amplification (RPA) assay for the isothermal amplification… CONTINUE READING

    Figures, Tables, and Topics from this paper.

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 28 REFERENCES
    DNA Detection Using Recombination Proteins
    723