Application of a lectin from the mushroom Polysporus squamosus for the histochemical detection of the NeuAcα2,6Galβ1,4Glc/GlcNAc sequence of N-linked oligosaccharides: a comparison with the Sambucus nigra lectin

@article{Toma2001ApplicationOA,
  title={Application of a lectin from the mushroom Polysporus squamosus for the histochemical detection of the NeuAcα2,6Galβ1,4Glc/GlcNAc sequence of N-linked oligosaccharides: a comparison with the Sambucus nigra lectin},
  author={Valeriu Toma and Christian Zuber and Harry C. Winter and Irwin J. Goldstein and J{\"u}rgen Roth},
  journal={Histochemistry and Cell Biology},
  year={2001},
  volume={116},
  pages={183-193}
}
The lectin from the mushroom Polysporus squamosus (PSL) has an extended carbohydrate combining site, which exhibits a high specificity and affinity toward the NeuAc5α2,6Galβ1,4Glc/GlcNAc trisaccharide sequence of asparagine-linked oligosaccharides. Therefore, PSL should be a superior reagent to the lectin from Sambucus nigra (SNA), which does not discriminate between α2,6-linked NeuAc5 present either in asparagine- or serine/threonine-linked oligosaccharides. We have prepared a digoxigenin… CONTINUE READING
15 Extracted Citations
54 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 15 extracted citations

Referenced Papers

Publications referenced by this paper.
Showing 1-10 of 54 references

Purification and molecular properties of a sialic acid - specific lectin from the slug Limax flavus

  • HQ Mo, HC Winter, IJ Goldstein
  • J Biol Chem
  • 2000

Different modes of sialyl - Tn expression during malignant transformation of human colonic mucosa

  • D Pousset, V Piller, N Bureaud, M Monsigny, F Piller
  • Glycoconj J
  • 1997

Purification and characterization of a Neu 5 Ac alpha 2 – 6 Gal beta 1 – 4 Glc / GlcNAc - specific lectin from the fruiting body of the polypore mushroom Polyporus squamosus

  • T Murayama, C Zuber, +4 authors J Roth
  • J Biol Chem
  • 1997

Similar Papers

Loading similar papers…