Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells.

@article{Wan2016ApplicationOV,
  title={Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells.},
  author={Yinglang Wan and Yiqun Xue and Ruili Li and Jinxing Lin},
  journal={Methods in molecular biology},
  year={2016},
  volume={1363},
  pages={123-32}
}
Variable angle total internal reflection fluorescence microscopy (VA-TIRFM) is an optical method to observe the molecular events occurring in an extremely thin region near the plasma membrane. Recently, the VA-TIRFM technique has been widely used to study fluorescently labeled target molecules in living animal and plant cells. Here, we describe the optical… CONTINUE READING