Application of Response Surface Methodology and Central Composite Design for 5P12-RANTES Expression in the <i>Pichia pastoris</i> System

Abstract

Pichia pastoris has demonstrated the ability to express high levels of recombinant heterologous proteins. Protein expression is enhanced during fermentation at high cell density. However, the level of expression is mainly regulated by fermentation operation factors. This research is directed to investigate the effect of methanol growth rate, temperature and… (More)

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