Application of Ion Torrent Sequencing to the Assessment of the Effect of Alkali Ballast Water Treatment on Microbial Community Diversity

@article{Fujimoto2014ApplicationOI,
  title={Application of Ion Torrent Sequencing to the Assessment of the Effect of Alkali Ballast Water Treatment on Microbial Community Diversity},
  author={Masanori Fujimoto and Gregory A. Moyerbrailean and Sifat Noman and Jason P. Gizicki and Michal L. Ram and P A Green and Jeffrey L. Ram},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
The impact of NaOH as a ballast water treatment (BWT) on microbial community diversity was assessed using the 16S rRNA gene based Ion Torrent sequencing with its new 400 base chemistry. Ballast water samples from a Great Lakes ship were collected from the intake and discharge of both control and NaOH (pH 12) treated tanks and were analyzed in duplicates. One set of duplicates was treated with the membrane-impermeable DNA cross-linking reagent propidium mono-azide (PMA) prior to PCR… 
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