Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.

@article{Bird2014ApplicationOI,
  title={Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.},
  author={Louise E. Bird and Heather Rada and John Flanagan and Jonathan M. Diprose and Robert J C Gilbert and Raymond J. Owens},
  journal={Methods in molecular biology},
  year={2014},
  volume={1116},
  pages={209-34}
}
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.