Application of HN(C)N to rapid estimation of 1J(N-C(alpha)) coupling constants correlated to psi torsion angles in proteins: implication to structural genomics.

@article{Bhavesh2003ApplicationOH,
  title={Application of HN(C)N to rapid estimation of 1J(N-C(alpha)) coupling constants correlated to psi torsion angles in proteins: implication to structural genomics.},
  author={Neel Sarovar Bhavesh and Amarnath Chatterjee and Sanjay C. Panchal and Ramakrishna V. Hosur},
  journal={Biochemical and biophysical research communications},
  year={2003},
  volume={311 3},
  pages={678-84}
}
We recently described a triple resonance experiment, HN(C)N, for sequential correlation of H(N) and 15N atoms in (15N, 13C) labeled proteins [J. Biomol. NMR. 20 (2001) 135]. Here, we describe an approach based on this experiment for estimation of one bond N-C(alpha) J-couplings in medium size labeled proteins, which seem to show good correlations with psi… CONTINUE READING