Application of FISH for in situ detection and quantification of DNA breakage.

@article{Fernndez1998ApplicationOF,
  title={Application of FISH for in situ detection and quantification of DNA breakage.},
  author={Jos{\'e} L. Fern{\'a}ndez and Vicente Goyanes and Juan Ramiro-D{\'i}az and Jaime Gos{\'a}lvez},
  journal={Cytogenetics and cell genetics},
  year={1998},
  volume={82 3-4},
  pages={251-6}
}
We describe a simple procedure that allows the use of fluorescence in situ hybridization (FISH) for in situ detection of DNA strand breaks in single cells (DBD-FISH: DNA Breakage Detection-FISH). After trapping within an agarose microgel, cells are incubated in an unwinding alkaline solution, deproteinized and dehydrated. Areas of single-stranded DNA are generated by the alkaline solution in proportion to the degree of DNA strand breakage. These then act as targets for FISH of whole genomic or… CONTINUE READING