Application of COMPOCHIP Microarray to Investigate the Bacterial Communities of Different Composts

@article{FrankeWhittle2008ApplicationOC,
  title={Application of COMPOCHIP Microarray to Investigate the Bacterial Communities of Different Composts},
  author={Ingrid H. Franke-Whittle and Brigitte Amalia Knapp and Jacques G. Fuchs and Ruediger Kaufmann and Heribert Insam},
  journal={Microbial Ecology},
  year={2008},
  volume={57},
  pages={510-521}
}
A microarray spotted with 369 different 16S rRNA gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. The microarray was tested with pure cultures, and of the 30,258 individual probe-target hybridization reactions performed, there were only 188 false positive (0.62%) and 22 false negative signals (0.07%). Labeled target DNA was prepared by polymerase chain reaction amplification of 16S rRNA genes using a Cy5-labeled… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 24 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 51 references

Sphingobacterium daejeonense sp. nov., isolated from a compost sample.

International journal of systematic and evolutionary microbiology • 2006
View 1 Excerpt

Similar Papers

Loading similar papers…