Application of Brønsted-type LFER in the study of the phospholipase C mechanism.

  title={Application of Br\onsted-type LFER in the study of the phospholipase C mechanism.},
  author={Cornelia Mihai and Alexander V Kravchuk and M Tsai and Karol S. Bruzik},
  journal={Journal of the American Chemical Society},
  volume={125 11},
Phosphatidylinositol-specific phospholipase C cleaves the phosphodiester bond of phosphatidylinositol to form inositol 1,2-cyclic phosphate and diacylglycerol. This enzyme also accepts a variety of alkyl and aryl inositol phosphates as substrates, making it a suitable model enzyme for studying mechanism of phosphoryl transfer by probing the linear free… CONTINUE READING