Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices.

@article{Wu2017ApplicationOA,
  title={Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices.},
  author={Qiuyu Wu and Alexander A. Chubykin},
  journal={Journal of visualized experiments : JoVE},
  year={2017},
  volume={125}
}
Whole-cell patch clamp is the gold-standard method to measure the electrical properties of single cells. However, the in vitro patch clamp remains a challenging and low-throughput technique due to its complexity and high reliance on user operation and control. This manuscript demonstrates an image-guided automatic patch clamp system for in vitro whole-cell patch clamp experiments in acute brain slices. Our system implements a computer vision-based algorithm to detect fluorescently labeled cells… CONTINUE READING