Application for PCR technology to subtractive cDNA cloning: identification of genes expressed specifically in murine plasmacytoma cells.

Abstract

We describe a simple method for preparing a renewable source of subtractive cDNA which can be used as a hybridization probe or as insert which can be cloned into a variety of convenient vectors. This has been done by ligating a double-stranded oligonucleotide to each end of double-stranded subtractive cDNA, and then using this oligonucleotide sequence to… (More)

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Cite this paper

@article{Timblin1990ApplicationFP, title={Application for PCR technology to subtractive cDNA cloning: identification of genes expressed specifically in murine plasmacytoma cells.}, author={C R Timblin and James F. Battey and Walter Michael Kuehl}, journal={Nucleic acids research}, year={1990}, volume={18 6}, pages={1587-93} }