Application and equivalence assessment for determining ethamsylate by using potassium ferricyanide as spectroscopic probe reagent.

Abstract

In this paper, a novel method has been established to determine ethamsylate using potassium ferricyanide as a spectroscopic probe reagent. It has been demonstrated that Fe(III) is reduced to Fe(II) by ethamsylate, and that the formed Fe(II) reacts with potassium ferricyanide to form soluble prussian blue (KFe(III)[Fe(II)(CN)(6)]). Beer's law is obeyed in the range of 0.16 - 24.00 µg mL(-1) with the molar absorption coefficient of 2.1 × 10(4) L mol(-1) cm(-1). The detection limit (3 σ/k) is 0.11 µg mL(-1). This method has been successfully applied to determine ethamsylate in pharmaceutical and serum samples with satisfactory results, and presented quite satisfactory credibility during method equivalence assessment.

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Cite this paper

@article{Liu2010ApplicationAE, title={Application and equivalence assessment for determining ethamsylate by using potassium ferricyanide as spectroscopic probe reagent.}, author={Litao Liu and Jing Li and Quan-min Li}, journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry}, year={2010}, volume={26 11}, pages={1163-7} }