Applicability of the 16S-23S rDNA internal spacer for PCR detection of the phytostimulatory PGPR inoculant Azospirillum lipoferum CRT1 in field soil.

@article{Baudoin2010ApplicabilityOT,
  title={Applicability of the 16S-23S rDNA internal spacer for PCR detection of the phytostimulatory PGPR inoculant Azospirillum lipoferum CRT1 in field soil.},
  author={Ez{\'e}kiel Baudoin and Olivier Couillerot and Stijn Spaepen and Y. Mo{\"e}nne-loccoz and Sylvie Nazaret},
  journal={Journal of applied microbiology},
  year={2010},
  volume={108 1},
  pages={25-38}
}
AIMS To assess the applicability of the 16S-23S rDNA internal spacer regions (ISR) as targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth-promoting rhizobacteria seed inoculant Azospirillum lipoferum CRT1 in soil. METHODS AND RESULTS Primer sets were designed after sequence analysis of the ISR of A. lipoferum CRT1 and Azospirillum brasilense Sp245. The primers fAZO/rAZO targeting the Azospirillum genus successfully yielded PCR amplicons (400-550 bp) from… CONTINUE READING

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