Appearance of three lymphokines in culture supernatants harvested at different times.

@article{Yong1982AppearanceOT,
  title={Appearance of three lymphokines in culture supernatants harvested at different times.},
  author={W. A. Yong and William J. Halliday},
  journal={Immunology letters},
  year={1982},
  volume={4 1},
  pages={21-6}
}
Peritoneal cells from mice with methylcholanthrene-induced tumours were reacted with the corresponding tumour extract (antigen) and supernatants were prepared after incubation times of 1, 3.5 and 24 h. All the supernatants were then tested simultaneously on the same pools of normal indicator cells for their effects on leucocyte adherence, E-rosette formation and macrophage migration. The leucocyte adherence inhibition factor (LAIF) was consistently detected only in the 1-h supernatant, E… CONTINUE READING