Appearance of crystalloid endoplasmic reticulum in compactin-resistant Chinese hamster cells with a 500-fold increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

@article{Chin1982AppearanceOC,
  title={Appearance of crystalloid endoplasmic reticulum in compactin-resistant Chinese hamster cells with a 500-fold increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase.},
  author={Daniel J. Chin and Kenneth L. Luskey and Richard G. W. Anderson and Jerry R Faust and Joseph L Goldstein and Michael Scott Brown},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 4},
  pages={1185-9}
}
We have developed a line of Chinese hamster ovary cells with a 500-fold increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase, the membrane-bound enzyme that controls cholesterol synthesis. This line, designated (UT-1, was obtained by stepwise adaptation of cells to growth in increasing concentrations of compactin, a competitive inhibitor of reductase. Reductase accounts for approximately 2% of total cell protein in UT-1 cells, as calculated from enzyme specific activity and by… CONTINUE READING