Appearance of actin microfilament 'twin peaks' in mitosis and their function in cell plate formation, as visualized in tobacco BY-2 cells expressing GFP-fimbrin.

@article{Sano2005AppearanceOA,
  title={Appearance of actin microfilament 'twin peaks' in mitosis and their function in cell plate formation, as visualized in tobacco BY-2 cells expressing GFP-fimbrin.},
  author={Toshio Sano and Takumi Higaki and Yoshihisa Oda and Tomomi Hayashi and Seiichiro Hasezawa},
  journal={The Plant journal : for cell and molecular biology},
  year={2005},
  volume={44 4},
  pages={595-605}
}
The actin cytoskeleton of higher plants plays an essential role in plant morphogenesis and in maintaining various cellular activities. In this study we established a tobacco BY-2 cell line, stably transformed with a GFP-fimbrin actin-binding domain (ABD) 2 construct, that allows visualization of actin microfilaments (MFs) in living cells. Using this cell line, designated BY-GF11, we performed time-sequential observations of MF dynamics during cell-cycle progression. Detailed analyses revealed… CONTINUE READING

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