Apparent association constants of tRNAs for the ribosomal A, P, and E sites.

@article{SchillingBartetzko1992ApparentAC,
  title={Apparent association constants of tRNAs for the ribosomal A, P, and E sites.},
  author={S Schilling-Bartetzko and Frangois Franceschi and Hans Sternbach and Knud H. Nierhaus},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 7},
  pages={4693-702}
}
Association constants for tRNA binding to poly(U) programmed ribosomes were assessed under standardized conditions with a single preparation of ribosomes, tRNAs, and elongation factors, respectively, at 15 and 10 mM Mg2+. Association constants were determined by Scatchard plot analysis (the constants are given in units of [10(7)/M] measured at 15 mM Mg2+): the ternary complex Phe-tRNA.elongation factor EF-Tu.GTP (12 +/- 3), Phe-tRNA (1 +/- 0.4), AcPhe-tRNA (0.7 +/- 0.3), and deacylated tRNA(Phe… CONTINUE READING

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