Apoptotic-like changes in equine spermatozoa separated by density-gradient centrifugation or after cryopreservation.

@article{Brum2008ApoptoticlikeCI,
  title={Apoptotic-like changes in equine spermatozoa separated by density-gradient centrifugation or after cryopreservation.},
  author={Andrea M. Brum and Khalida Sabeur and Barry A Ball},
  journal={Theriogenology},
  year={2008},
  volume={69 9},
  pages={
          1041-55
        }
}
The objective was to evaluate apoptotic markers in ejaculated equine spermatozoa after separation by density-gradient centrifugation and after cryopreservation. Subpopulations of percoll-separated equine spermatozoa differed (P<0.05) in the percentage of live, caspase-activated spermatozoa (2.9+/-0.7% vs 14.2+/-6.4%; mean+/-S.E.M.), low mitochondrial membrane potential (MMP; 6.8+/-1.1 vs 23.8+/-3.7), altered plasma membrane permeability (1.3+/-0.2 vs 3.0+/-0.5), DNA fragmentation (2.0+/-1.3 vs… CONTINUE READING
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