Apoptotic events induced by the phosphatase inhibitor okadaic acid in normal human lung fibroblasts.

@article{Leira2001ApoptoticEI,
  title={Apoptotic events induced by the phosphatase inhibitor okadaic acid in normal human lung fibroblasts.},
  author={Francisco Jos{\'e} Sequeiro Leira and Juan Manuel Vieites and Mercedes Rodr{\'i}guez Vieytes and Luis M. Botana},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={2001},
  volume={15 3},
  pages={
          199-208
        }
}
We have studied different biochemical indicators of apoptosis in okadaic acid-treated normal human lung fibroblasts (NHLF). Apoptosis was identified by fluorimetric microplate measurements of DNA content, caspase-3 activation and changes in mitochondrial and plasma membrane after 1-48-h treatments with 1-1000 nM okadaic acid. Cells exposed to okadaic acid showed activation of caspase-3, decreased DNA content (<50% of controls at >100 nM okadaic acid after 12 h of incubation) and translocation… CONTINUE READING

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Okadaic acid induces apoptosis through the PKR, NF-κB and caspase pathway in human osteoblastic osteosarcoma MG63 cells.

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