Apoptotic characteristics of cell death and the neuroprotective effect of homocarnosine on pheochromocytoma PC12 cells exposed to ischemia.

@article{Tabakman2004ApoptoticCO,
  title={Apoptotic characteristics of cell death and the neuroprotective effect of homocarnosine on pheochromocytoma PC12 cells exposed to ischemia.},
  author={Rinat Tabakman and Hao Jiang and Robert A. Levine and Ron Kohen and Philip Lazarovici},
  journal={Journal of neuroscience research},
  year={2004},
  volume={75 4},
  pages={
          499-507
        }
}
We recently improved an in vitro ischemic model, using PC12 neuronal cultures exposed to oxygen-glucose deprivation (OGD) for 3 hr in a special device, followed by 18 hr of reoxygenation. The cell death induced in this ischemic model was evaluated by a series of markers: lactate dehydrogenase (LDH) release, caspase-3 activation, presence of cyclin D1, cytochrome c leakage from the mitochondria, BAX cellular redistribution, cleavage of poly (ADP-ribose) polymerase (PARP) to an 85-kDa apoptotic… CONTINUE READING
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