Apoptotic cell death and function of cryopreserved porcine hepatocytes in a bioartificial liver.

@article{Matsushita2003ApoptoticCD,
  title={Apoptotic cell death and function of cryopreserved porcine hepatocytes in a bioartificial liver.},
  author={Takakazu Matsushita and Toshikazu Yagi and Joseph A Hardin and Jennifer D Cragun and Frank W Crow and H. R. Bergen and Gregory J. Gores and Scott L. Nyberg},
  journal={Cell transplantation},
  year={2003},
  volume={12 2},
  pages={109-21}
}
We have previously shown that cryopreservation leads to increased apoptotic death of porcine hepatocytes intended for use in a bioartificial liver (BAL). This study was designed to determine if a broad-spectrum caspase inhibitor, IDN-1965, reduced apoptosis and increased function of cryopreserved porcine hepatocytes in static culture or in a BAL. Porcine hepatocytes were studied immediately after isolation and after 2 weeks of cryopreservation in liquid nitrogen using medium supplemented with… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Best practices for cryopreserving, thawing, recovering, and assessing cells

In Vitro Cellular & Developmental Biology - Animal • 2017
View 2 Excerpts

Similar Papers

Loading similar papers…