Apoptosis is induced in leishmanial cells by a novel protein kinase inhibitor withaferin A and is facilitated by apoptotic topoisomerase I–DNA complex

@article{Sen2007ApoptosisII,
  title={Apoptosis is induced in leishmanial cells by a novel protein kinase inhibitor withaferin A and is facilitated by apoptotic topoisomerase I–DNA complex},
  author={Nilkantha Sen and Bijoylaxhmi Banerjee and Benu Brata Das and Ashish Ganguly and Tanusree Sen and Shahidul Pramanik and Saikat Mukhopadhyay and Hemanta Kumar Majumder},
  journal={Cell Death and Differentiation},
  year={2007},
  volume={14},
  pages={358-367}
}
Protein kinase C (PKC) is an important constituent of the signaling pathways involved in apoptosis. We report here that like staurosporine, withaferin A is a potent inhibitor of PKC. In Leishmania donovani, the inhibition of PKC by withaferin A causes depolarization of ΔΨm and generates ROS inside cells. Loss of ΔΨm leads to the release of cytochrome c into the cytosol and subsequently activates caspase-like proteases and oligonucleosomal DNA cleavage. Moreover, in treated cells, oxidative DNA… CONTINUE READING

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