Apoptosis in patients with myelodysplastic syndromes: differential involvement of marrow cells in ‘good’ versus ‘poor’ prognosis patients and correlation with apoptosis-related genes

@article{Tsoplou1999ApoptosisIP,
  title={Apoptosis in patients with myelodysplastic syndromes: differential involvement of marrow cells in ‘good’ versus ‘poor’ prognosis patients and correlation with apoptosis-related genes},
  author={P Tsoplou and Alexandra Kouraklis-Symeonidis and Eleni Thanopoulou and Panagiotis Zikos and V. Orphanos and N Zoumbos},
  journal={Leukemia},
  year={1999},
  volume={13},
  pages={1554-1563}
}
Apoptosis has been implicated in the pathogenesis of marrow failure in MDS and the coexistence of marrow hypercellularity along with blood cytopenias was seen as evidence of extreme cell death of mainly mature cells in the marrow (ineffective hematopoiesis). We investigated apoptosis in 53 patients with MDS, by using single-step DNA extraction and gel electrophoresis and then by separating fresh marrow mononuclear cells in CD34+ and CD34− populations and in situ single cell evaluation of the… CONTINUE READING