Apoptosis in developing anthers and the role of ABA in this process during androgenesis in Hordeum vulgare L.

  title={Apoptosis in developing anthers and the role of ABA in this process during androgenesis in Hordeum vulgare L.},
  author={Mei Wang and Sietske Hoekstra and Sandra van Bergen and Gerda Lamers and Berry J. Oppedijk and Maurice W. van der Heijden and Wessel de Priester and Rob A. Schilperoort},
  journal={Plant Molecular Biology},
Intra-nucleosomal cleavage of DNA into fragments of about 200 bp was demonstrated to occur in developing anthers, in which microspores had developed into the mid-late to late uni-nucleate stage in situ, i.e. at the verge of mitosis. The same was observed, but to a much larger extent, if these anthers were pre- treated by a hyper-osmotic shock. Pretreatment of anthers before the actual culture of microspores was required for optimal androgenesis of microspores. The use of the TUNEL reaction… CONTINUE READING


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