Apoptosis and ultrastructural assessment after cryopreservation of whole human ovaries with their vascular pedicle.

@article{MartinezMadrid2007ApoptosisAU,
  title={Apoptosis and ultrastructural assessment after cryopreservation of whole human ovaries with their vascular pedicle.},
  author={Belen Martinez-Madrid and Alessandra Camboni and M V Dolmans and Stefania Annarita Nottola and Anne van Langendonckt and Jacques Donnez},
  journal={Fertility and sterility},
  year={2007},
  volume={87 5},
  pages={1153-65}
}
OBJECTIVE To investigate possible damage caused by freeze-thawing whole human ovaries. DESIGN Prospective experimental study. SETTING Academic gynecology research unit in a university hospital. PATIENT(S) Ovaries were obtained from three women (aged 29-36 years). INTERVENTION(S) Ovaries were perfused and bathed in cryoprotective solution, and slow freezing was performed. Rapid thawing was achieved by perfusion and bathing with a decreased sucrose gradient. MAIN OUTCOME MEASURE(S… CONTINUE READING
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