Apolipoprotein E1 Lys-146----Glu with type III hyperlipoproteinemia.

  title={Apolipoprotein E1 Lys-146----Glu with type III hyperlipoproteinemia.},
  author={Kazuhiro Moriyama and Jun Sasaki and Akira Hatakeyama Osamu Keishima Kiyotaka Nakagawa Yoshia Matsunaga and Fumiko Arakawa and Yasunobu Takada and Kana Araki and Shuichi Kaneko and Kikuo Arakawa},
  journal={Biochimica et biophysica acta},
  volume={1128 1},
During the screening of samples obtained from 5 individuals with type III hyperlipidemia, we identified a variant of apolipoprotein (apo) E which exhibited a discrepancy in apo E phenotype showing the E3/E1 isoform on isoelectric focusing (IEF) analysis and E3/E3 on gene analysis. Sequence analysis of the DNA of the proband that was amplified by PCR and subcloned, revealed a single substitution of one lysine (AAG) for one glutamic acid (GAG) at position 146, thereby adding two negatively… CONTINUE READING