Apolipoprotein C-II deficiency associated with nonfunctional mutant forms of apolipoprotein C-II.

Abstract

Two previously unidentified apolipoproteins (apo) designated apo C-II-X and C-II-Y have been found in plasma of homozygotes and obligate heterozygotes of a family with apo C-II deficiency. Because the plasma of homozygotes do not activate lipoprotein lipase, apo C-II-X and C-II-Y are apparently nonfunctional. These apolipoproteins have isoelectric focusing points of 5.15 and 5.54, respectively, compared with 4.88 and 4.74 for the known isomorphs, C-II-1 and C-II-2, respectively. They have approximately similar molecular weights to apo C-II-1 and C-II-2 on two-dimensional sodium dodecyl sulphate-glycerol-polyacrylamide slab gel electrophoresis. They do not form insoluble antigen-antibody complexes with antibodies to apo C-II in single antibody immunodiffusion or electroimmunoassay systems. However, using a double-antibody technique in which immunoblotting is coupled with polyacrylamide isoelectric focusing slab gel electrophoresis, apo C-II-1, C-II-2, C-II-X, and C-II-Y have similar reactivity with antibodies to apo C-II. In this family the presence of apo C-II-X and C-II-Y discriminates obligate heterozygotes from normal subjects.

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@article{Maguire1984ApolipoproteinCD, title={Apolipoprotein C-II deficiency associated with nonfunctional mutant forms of apolipoprotein C-II.}, author={Graham F. Maguire and J. Alick Little and Gary Kakis and W. Carl Breckenridge}, journal={Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire}, year={1984}, volume={62 9}, pages={847-52} }