Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons.

  title={Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons.},
  author={J. Habash and T. Boggon and J. Raftery and N. Chayen and P. F. Zagalsky and J. Helliwell},
  journal={Acta crystallographica. Section D, Biological crystallography},
  volume={59 Pt 7},
Models of apocrustacyanin C(1) were refined against X-ray data recorded on Bending Magnet 14 at the ESRF to resolutions of 1.85 and 2 A from a space-grown and an earth-grown crystal, respectively, both using vapour-diffusion crystal-growth geometry. The space crystals were grown in the APCF on the NASA Space Shuttle. The microgravity crystal growth showed a cyclic nature attributed to Marangoni convection, thus reducing the benefits of the microgravity environment, as reported previously… Expand
Macromolecular crystallization in microgravity
Density difference fluid flows and sedimentation of growing crystals are greatly reduced when crystallization takes place in a reduced gravity environment. In the case of macromolecularExpand
Microgravity as an environment for macromolecular crystallization – an outlook in the era of space stations and commercial space flight
ABSTRACT In 2005 we reviewed microgravity for macromolecular crystallization, four years after the final flight of the Space Shuttle Orbiter, and five years before the first commercial flight to theExpand
An evaluation review of the prediction of protonation states in proteins versus crystallographic experiment
The known protonation states of protein crystal structures obtained using X-ray and neutron crystallographic data, and including relevant NMR derived experimental information, have been predictedExpand
1.4 X-Ray Crystallography: Crystallization
Structure determination of biological macromolecules using x-ray crystallography has been greatly improved in recent years through the development of a number of key technologies and betterExpand
Protein Crystallization on the International Space Station ISS
Already early protein crystallization experiments in space indicated that more extended crystallization periods, beyond the flight durations of shuttle missions or unmanned orbiters, will beExpand
Prawn lipocalin: characterization of a color shift induced by gene knockdown and ligand binding assay.
The lipocalin family of proteins functions in the transport of steroids, carotenoids, retinoids, and other small hydrophobic molecules. Recently, a lipocalin (MrLC) was isolated from the prawnExpand
Evolution of a novel carotenoid-binding protein responsible for crustacean shell color.
It is submitted that the origin of the CRCN gene family early in the evolution of malacostracan crustaceans significantly contributed to the success of this group of arthropods. Expand
Rapid expansion of pigmentation genes in penaeid shrimp with absolute preservation of function
The regulation and molecular evolution of pigmentation in crustaceans is more complex than initially thought, with multiple lineage-specific duplications and functional redundancy in shrimp pigmentation genes. Expand
Ultraviolet Radiation Tolerance in High Elevation Copepods from the Rocky Mountains of Colorado, USA
Diaptomid color morph may be an important indicator of UV conditions in high elevation lentic ecosystems and has a greater effect on resistance to UV exposure than other color morphs. Expand
From Target identification to Drug Development in Space: Using the Microgravity Assist.
  • Martin Braddock
  • Medicine, Computer Science
  • Current drug discovery technologies
  • 2019
A review of studies conducted on Space Shuttle missions, the International Space Station and other craft have had direct benefit for drug development programmes such as those directed against reducing bone and muscle loss or increasing bone formation. Expand


Protein and virus crystal growth on international microgravity laboratory-2.
X-ray diffraction analysis demonstrated that both crystal forms of canavalin and the cubic STMV crystals diffracted to significantly higher resolution and had superior diffraction properties as judged by relative Wilson plots. Expand
CCD video observation of microgravity crystallization: apocrustacyanin C1
Abstract Apocrustacyanin C 1 has been crystallized in the vapour-diffusion apparatus of ESA's Advanced Protein Crystallization Facility (APCF) on-board the NASA space shuttle STS-65 InternationalExpand
Materials: protein single crystal growth under microgravity.
Crystals formed by salting out from solutions kept free of convection were 27 and 1000 times larger in volume, respectively, than those produced in the same apparatus but exposed to terrestrial gravitation. Expand
[20] Processing of X-ray diffraction data collected in oscillation mode.
The methods presented in the chapter have been applied to solve a large variety of problems, from inorganic molecules with 5 A unit cell to rotavirus of 700 A diameters crystallized in 700 × 1000 × 1400 A cell. Expand
Trends and challenges in experimental macromolecular crystallography.
Macromolecular X-ray crystallography underpins the vigorous field of structural molecular biology having yielded many protein, nucleic acid and virus structures in fine detail, and from such structures the functional details can be inferred, such as the biological chemistry of enzyme reactivity. Expand
Recent advances in the microgravity crystallization of biological macromolecules.
The status of microgravity research in the crystallization of biological macromolecules and the exploration of the flash-frozen-samples-Dewar approach for multiple crystallizations is presented. Expand
Improved methods for building protein models in electron density maps and the location of errors in these models.
Map interpretation remains a critical step in solving the structure of a macromolecule. Errors introduced at this early stage may persist throughout crystallographic refinement and result in anExpand