Apicidin F: Characterization and Genetic Manipulation of a New Secondary Metabolite Gene Cluster in the Rice Pathogen Fusarium fujikuroi

@article{Niehaus2014ApicidinFC,
  title={Apicidin F: Characterization and Genetic Manipulation of a New Secondary Metabolite Gene Cluster in the Rice Pathogen Fusarium fujikuroi},
  author={Eva-Maria Niehaus and Slavica Janevska and Katharina Walburga von Bargen and Christian M. K. Sieber and Henning Harrer and Hans-Ulrich Humpf and Bettina Tudzynski},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
The fungus F. fujikuroi is well known for its production of gibberellins causing the ‘bakanae’ disease of rice. Besides these plant hormones, it is able to produce other secondary metabolites (SMs), such as pigments and mycotoxins. Genome sequencing revealed altogether 45 potential SM gene clusters, most of which are cryptic and silent. In this study we characterize a new non-ribosomal peptide synthetase (NRPS) gene cluster that is responsible for the production of the cyclic tetrapeptide… Expand
Secondary metabolism in Fusarium fujikuroi: strategies to unravel the function of biosynthetic pathways
TLDR
It is demonstrated that deletion and over-expression of histone-modifying genes is a powerful tool to activate silent gene clusters and to discover their products, and several examples where linking known metabolites to the respective biosynthetic genes has been achieved. Expand
Comparative genomics of geographically distant Fusarium fujikuroi isolates revealed two distinct pathotypes correlating with secondary metabolite profiles
TLDR
Analysis of additional field isolates confirmed the strong correlation between the pathotype (bakanae or stunting/withering), and the ability to produce either GAs or fumonisins. Expand
The global regulator FfSge1 is required for expression of secondary metabolite gene clusters but not for pathogenicity in Fusarium fujikuroi.
The plant pathogenic fungus Fusarium fujikuroi is the causal agent of bakanae disease on rice due to its ability to produce gibberellins. Besides these phytohormones, F. fujikuroi is able to produceExpand
Analysis of the global regulator Lae1 uncovers a connection between Lae1 and the histone acetyltransferase HAT1 in Fusarium fujikuroi
TLDR
A deeper insight into the genome-wide regulatory impact of Lae1 on secondary metabolism is presented and over-expression of lae1 resulted in de-repression of GA biosynthetic genes under otherwise repressing high nitrogen conditions demonstrating that the nitrogen repression is overcome. Expand
Advances in linking polyketides and non-ribosomal peptides to their biosynthetic gene clusters in Fusarium
TLDR
The aim of this review is to describe the different methods that have successfully been used throughout the genus Fusarium to identify the products of novel biosynthetic pathways, and encourage the development of new techniques and strategies to provide access to the many unknown polyketide and non-ribosomal peptide products that await discovery in FUSarium. Expand
Interplay between pathway-specific and global regulation of the fumonisin gene cluster in the rice pathogen Fusarium fujikuroi
TLDR
It is demonstrated that both GATA-type transcription factors are essential for full activation of the FUM gene cluster and loss of one of them obstructs the pathway-specific transcription factor Fum21 to fully activate expression of FUM cluster genes. Expand
Isolation and Structure Elucidation of Fujikurins A-D: Products of the PKS19 Gene Cluster in Fusarium fujikuroi.
TLDR
The structure elucidation of the products of the cryptic and silent PKS19 gene cluster that were recently identified (fujikurins A-D) shows tautomeric 1,3-diketo elements, which could be confirmed using quantum chemical calculations. Expand
Gibepyrone Biosynthesis in the Rice Pathogen Fusarium fujikuroi Is Facilitated by a Small Polyketide Synthase Gene Cluster*
TLDR
Evidence is obtained that Gpy2 is involved in gene regulation as it represses GPY1 gene expression and gibepyrone A production was enhanced both extra- and intracellularly in Δgpy2 mutants. Expand
Sound of silence: the beauvericin cluster in Fusarium fujikuroi is controlled by cluster-specific and global regulators mediated by H3K27 modification.
TLDR
A novel peak was identified in ΔHDA1, which was identified as beauvericin (BEA), and going in line with a 1000-fold increased BEA production, the respective non-ribosomal peptide synthetase (NRPS)-encoding gene (BE a1), as well as two adjacent genes (BEa2-BEA3), were significantly up-regulated in Δ HDA1 compared to the wild type. Expand
The Genome of the Generalist Plant Pathogen Fusarium avenaceum Is Enriched with Genes Involved in Redox, Signaling and Secondary Metabolism
TLDR
Many protein families are expanded in F. avenaceum, such as transcription factors, and proteins involved in redox reactions and signal transduction, suggesting evolutionary adaptation to a diverse and cosmopolitan ecology. Expand
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