Apical sorting of a voltage- and Ca2+-activated K+ channel alpha -subunit in Madin-Darby canine kidney cells is independent of N-glycosylation.

@article{BravoZehnder2000ApicalSO,
  title={Apical sorting of a voltage- and Ca2+-activated K+ channel alpha -subunit in Madin-Darby canine kidney cells is independent of N-glycosylation.},
  author={Marcela Bravo-Zehnder and Patricio Orio and Andr{\'e}s Norambuena and Martin Wallner and Pratap Meera and Ligia Toro and Ram{\'o}n Latorre and Alfonso Gonz{\'a}lez},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 24},
  pages={13114-9}
}
The voltage- and Ca(2+)-activated K(+) (K(V,Ca)) channel is expressed in a variety of polarized epithelial cells seemingly displaying a tissue-dependent apical-to-basolateral regionalization, as revealed by electrophysiology. Using domain-specific biotinylation and immunofluorescence we show that the human channel K(V,Ca) alpha-subunit (human Slowpoke channel, hSlo) is predominantly found in the apical plasma membrane domain of permanently transfected Madin-Darby canine kidney cells. Both the… CONTINUE READING