Apical membrane chloride channels in a colonic cell line activated by secretory agonists.

@article{Halm1988ApicalMC,
  title={Apical membrane chloride channels in a colonic cell line activated by secretory agonists.},
  author={Dan R. Halm and G R Rechkemmer and Robert A. Schoumacher and Raymond A Frizzell},
  journal={The American journal of physiology},
  year={1988},
  volume={254 4 Pt 1},
  pages={C505-11}
}
We characterized the anion channel responsible for the increase in apical membrane Cl secretion using a model salt-secreting epithelium, the T84 colonic cell line. The adenosine 3',5'-cyclic monophosphate (cAMP)-mediated secretagogues, prostaglandin E2, forskolin, and 8-bromo-cAMP, evoked activity of an outwardly rectifying Cl channel in previously quiet cell-attached membrane patches. The channel remained active in excised, inside-out membranes, where its single-channel conductance was 40-45… CONTINUE READING