Aphid transmission of cauliflower mosaic virus requires the viral PIII protein.

  title={Aphid transmission of cauliflower mosaic virus requires the viral PIII protein.},
  author={V{\'e}ronique Leh and Emmanuel Jacquot and Ang{\`e}le Geldreich and Thomas Hermann and Denis Leclerc and Martine C{\'e}rutti and Pierre Yot and Mario Keller and St{\'e}phane Blanc},
  journal={The EMBO journal},
  volume={18 24},
The open reading frame (ORF) III product (PIII) of cauliflower mosaic virus is necessary for the infection cycle but its role is poorly understood. We have used in vitro protein binding ('far Western') assays to demonstrate that PIII interacts with the cauliflower mosaic virus (CaMV) ORF II product (PII), a known aphid transmission factor. Aphid transmission of purified virions of the PII-defective strain CM4-184 was dependent upon added PII, but complementation was efficient only in the… CONTINUE READING


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