Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells

@article{Ding2012AntitumorEO,
  title={Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Gu{\'e}rin vaccine on bladder cancer cells},
  author={Guo-qing Ding and Yan-lan Yu and Zhoujun Shen and Xie-lai Zhou and Shanwen Chen and Guo-dong Liao and Yue Zhang},
  journal={Journal of Zhejiang University SCIENCE B},
  year={2012},
  volume={13},
  pages={335-341}
}
Our objective was to construct a recombinant bacillus Calmette-Guérin vaccine (rBCG) that secretes human interferon-alpha 2b (IFNα-2b) and to study its immunogenicity and in vitro antitumor activity against human bladder cancer cell lines T24 and T5637. The signal sequence BCG Ag85B and the gene IFNα-2b were amplified from the genome of BCG and human peripheral blood, respectively, by polymerase chain reaction (PCR). The two genes were cloned in Escherichia coli-BCG shuttle-vector pMV261 to… CONTINUE READING
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