Antitumor effects of cationic synthetic peptides derived from Lys49 phospholipase A2 homologues of snake venoms

@article{Araya2007AntitumorEO,
  title={Antitumor effects of cationic synthetic peptides derived from Lys49 phospholipase A2 homologues of snake venoms},
  author={C. Araya and B. Lomonte},
  journal={Cell Biology International},
  year={2007},
  volume={31}
}
The effects of two cationic synthetic peptides, derived from the C‐terminal region of Lys49 phospholipase A2 homologues from snake venoms, upon various murine tumor cell lines (B16 melanoma, EMT6 mammary carcinoma, S‐180 sarcoma, P3X myeloma, tEnd endothelial cells) were evaluated. The peptides are 13‐mers derived from Agkistrodon piscivorus piscivorus Lys49 PLA2 (p‐AppK: KKYKAYFKLKCKK) and Bothrops asper Lys49 myotoxin II (pEM‐2[d]: KKWRWWLKALAKK), respectively, in the latter case with slight… Expand
Antitumor effects of snake venom chemically modified Lys49 phospholipase A2-like BthTX-I and a synthetic peptide derived from its C-terminal region.
TLDR
Injection of the modified protein or the peptide in mice, 5 days after transplantation of S180 tumor cells, reduced 30 and 36% of the tumor size on day 14th and 76 and 79% on day 60th, respectively, when compared to the untreated control group. Expand
Potential use of 13-mer peptides based on phospholipase and oligoarginine as leishmanicidal agents.
TLDR
The results suggest that short peptides based on phospholipase toxins are potential scaffolds for development of antileishmanial candidates and specific amino acid substitutions may enhance the antiparasitic action of these cationic peptides, encouraging their future biomedical applications. Expand
Antitumoral Activity of Snake Venom Proteins: New Trends in Cancer Therapy
TLDR
Higher cytotoxic and cytostatic activities upon tumor cells than normal cells suggest the possibility for clinical applications. Expand
Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines
TLDR
Assessment of in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human and murine tumor cell lines suggest that BthTX-I presents antitumor properties that may be useful for developing new therapeutic strategies against cancer. Expand
Identification of the Molecular Determinants of the Antibacterial Activity of LmutTX, a Lys49 Phospholipase A2 Homologue Isolated from Lachesis muta muta Snake Venom (Linnaeus, 1766)
TLDR
This study describes for the first time the isolation of a Lys49 PLA2 from Lachesis snake venom and shows that peptides from specific regions of the sequence may constitute new sources of molecules with biotechnological potential. Expand
Myotoxic phospholipases A2 isolated from Bothrops brazili snake venom and synthetic peptides derived from their C-terminal region: Cytotoxic effect on microorganism and tumor cells
TLDR
It is demonstrated that MTXs and cationic synthetic peptides derived from their 115-129 C-terminal region displayed cytotoxic activity on human T-cell leukemia (JURKAT) lines and microbicidal effects against Escherichia coli, Candida albicans and Leishmania sp. Expand
In vitro studies on Bothrops venoms cytotoxic effect on tumor cells.
TLDR
All Bothrops venoms tested showed cytotoxicity against tumor cell lines through inducing of necrosis and apoptosis. Expand
Phospholipase A2 from krait Bungarus fasciatus venom induces human cancer cell death in vitro
TLDR
Flow cytometry analysis of MCF7 cells stained with propidium iodide and Annexin V conjugated with allophycocyanin showed that a probable mechanism of cell death is apoptosis, the first krait phospholipase A2 manifesting the cytotoxicity for cancer cells. Expand
MVL-PLA2, a phospholipase A2 from Macrovipera lebetina transmediterranea venom, inhibits tumor cells adhesion and migration.
  • A. Bazaa, J. Luis, +6 authors N. Marrakchi
  • Biology, Medicine
  • Matrix biology : journal of the International Society for Matrix Biology
  • 2009
TLDR
It is demonstrated for the first time that the anti-tumor effect of MVL-PLA2 was mediated by alpha5beta1 and alphav-containing integrins, suggesting the presence of 'pharmacological sites' distinct from the catalytic site in snake venom phospholipase A2. Expand
Antitumor Effect of Cationic INKKI Peptide from Bovine β-Casein on Melanoma B16F10
TLDR
In conclusion, it is shown that INKKI is a peptide that could be considered a new putative candidate development to anticancer therapy drug. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 39 REFERENCES
Bactericidal and Antiendotoxic Properties of Short Cationic Peptides Derived from a Snake Venom Lys49 Phospholipase A2
TLDR
It is demonstrated that phospholipase A2-derived peptides may have the potential to counteract microbial infections and encourage further evaluations of their actions in vivo. Expand
Comparative study of synthetic peptides corresponding to region 115-129 in Lys49 myotoxic phospholipases A2 from snake venoms.
TLDR
Peptide AppK synthesized with D-amino acids retained both activities of the natural L-enantiomer, suggesting that its mechanism of action does not involve the recognition of a proteic receptor/acceptor site on muscle cells, but possibly the binding to other structures, such as negatively-charged membrane phospholipids. Expand
A Lys49 phospholipase A2 homologue from Bothrops asper snake venom induces proliferation, apoptosis and necrosis in a lymphoblastoid cell line
TLDR
It is concluded that a single phospholipase A2 homologue can induce markedly different effects on a single cell line, depending on the concentration used, an observation that may have implications for the action of this type of venom component in vivo. Expand
Antimicrobial activity of myotoxic phospholipases A2 from crotalid snake venoms and synthetic peptide variants derived from their C-terminal region.
TLDR
The bactericidal and anti-endotoxic properties of pEM-2, combined with its relatively low toxicity towards eukaryotic cells, highlight it as a promising candidate for further evaluation of its antimicrobial potential in vivo. Expand
Bactericidal activity of Lys49 and Asp49 myotoxic phospholipases A2 from Bothrops asper snake venom--synthetic Lys49 myotoxin II-(115-129)-peptide identifies its bactericidal region.
TLDR
Bacterial LPS chimeras indicated that LPS is a relevant target for myotoxin II-(115-129)-peptide, which represents a group-II PLA2 with a direct bactericidal effect that is independent of an intrinsic enzymatic activity, but adscribed to the presence of a short cluster of basic/hydrophobic amino acids near its C-terminal loop. Expand
Enhanced antitumor activity and selectivity of lactoferrin-derived peptides.
TLDR
Quantitative structure-activity relationship studies showed that certain structural parameters affected toxicity against the tumor cell lines more than against fibroblasts. Expand
New lytic peptides based on the D,L-amphipathic helix motif preferentially kill tumor cells compared to normal cells.
TLDR
The simple composition of the diastereomeric peptides and their stability regarding enzymatic degradation by serum components make them excellent candidates for new chemotherapeutic drugs. Expand
A Novel Lytic Peptide Composed of dl-Amino Acids Selectively Kills Cancer Cells in Culture and in Mice*
TLDR
A short cationic diastereomeric peptide composed of d- and l-leucines, lysines, and arginines that has selective toxicity toward cancer cells and significantly inhibits lung metastasis formation in mice with no detectable side effects is designed. Expand
Identification of the myotoxic site of the Lys49 phospholipase A(2) from Agkistrodon piscivorus piscivorus snake venom: synthetic C-terminal peptides from Lys49, but not from Asp49 myotoxins, exert membrane-damaging activities.
TLDR
These observations unequivocally identify the sequence 115-129 (KKYKAYFKLKCKK) of the Lys49 PLA(2) of A. p. Expand
An overview of lysine-49 phospholipase A2 myotoxins from crotalid snake venoms and their structural determinants of myotoxic action.
TLDR
It is proposed that all the toxic activities of Lys49 PLA2s are related to their ability to destabilize natural and artificial membranes, using a cationic/hydrophobic effector site located at their C-terminal loop. Expand
...
1
2
3
4
...