Antitumor activity of L-asparaginase from Thermus thermophilus.

  title={Antitumor activity of L-asparaginase from Thermus thermophilus.},
  author={Agathi A. Pritsa and Konstantinos T. Papazisis and Alexandros H. Kortsaris and George D. Geromichalos and Kyriakidis},
  journal={Anti-cancer drugs},
  volume={12 2},
L-asparaginase (EC was purified to homogeneity from Thermus thermophilus. The apparent molecular mass of L-asparaginase was found to be 33 kDa by SDS-PAGE, whereas by Sephacryl S-300 superfine column it was found to be 200 kDa, indicating that the enzyme in the native stage acts as hexamer. It is a thermostable enzyme and keeps all of its activity at 80 degrees C for 10 min. The antiproliferative activity of the purified L-asparaginase from T. thermiphilus was tested against the… CONTINUE READING