Antisera to glutathione: characterization and immunocytochemical application to the rat cerebellum.

@article{Hjelle1994AntiseraTG,
  title={Antisera to glutathione: characterization and immunocytochemical application to the rat cerebellum.},
  author={Ole Petter Hjelle and Farrukh Abbas Chaudhry and Ole Petter Ottersen},
  journal={The European journal of neuroscience},
  year={1994},
  volume={6 5},
  pages={793-804}
}
Rabbits were immunized with reduced glutathione (gamma-glutamyl-cysteinyl-glycine) coupled to bovine serum albumin by glutaraldehyde or a mixture of glutaraldehyde and formaldehyde. The antisera that were formed were tested qualitatively, by screening them against more than 50 amino acids and peptide conjugates that had been immobilized on cellulose discs (spot test), and quantitatively, by immunogold analysis of test conjugates that had been embedded in an epoxy resin. It was shown that the… CONTINUE READING

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