Antisense regulation of the rice waxy gene expression using a PCR-amplified fragment of the rice genome reduces the amylose content in grain starch

@article{Shimada1993AntisenseRO,
  title={Antisense regulation of the rice waxy gene expression using a PCR-amplified fragment of the rice genome reduces the amylose content in grain starch},
  author={Hiroaki Shimada and Yuichi Tada and Tsutomu Kawasaki and Tatsuhito Fujimura},
  journal={Theoretical and Applied Genetics},
  year={1993},
  volume={86},
  pages={665-672}
}
The waxy gene encodes a granule-bound starch synthase. A 1.0-kb portion of the sequence of the rice waxy gene, which includes the region between exon 4 and exon 9, was inserted in an antisense orientation between the 35 S promoter and the GUS gene of pBI221. The resultant plasmid, pWXA23, was introduced into rice protoplasts by electroporation. GUS activity was clearly detected in derived callus lines, suggesting that the antisense component of the fusion gene was also expressed. Transgenic… CONTINUE READING
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