Antisense phenotypes reveal a role for SHY, a pollen-specific leucine-rich repeat protein, in pollen tube growth.

@article{Guyon2004AntisensePR,
  title={Antisense phenotypes reveal a role for SHY, a pollen-specific leucine-rich repeat protein, in pollen tube growth.},
  author={Virginie N. Guyon and W. Grace Tang and Maurilia Maria Monti and Alessandro Raiola and Giulia de Lorenzo and Sheila McCormick and Loverine P. Taylor},
  journal={The Plant journal : for cell and molecular biology},
  year={2004},
  volume={39 4},
  pages={643-54}
}
SHY, a pollen-specific gene identified in a screen for genes upregulated at pollen germination, encodes a leucine-rich repeat (LRR) protein that is predicted to be secreted. To test if SHY plays an important role during pollen germination, we generated transgenic plants expressing an antisense (AS) copy of the SHY cDNA in pollen. Primary transformants exhibited poor seed set, but homozygous lines could be identified. In these lines, nearly all pollen tubes failed to reach the ovules; tube… CONTINUE READING

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