Antisense oligonucleotide containing an internal, non-nucleotide-based linker promote site-specific cleavage of RNA.

@article{Reynolds1996AntisenseOC,
  title={Antisense oligonucleotide containing an internal, non-nucleotide-based linker promote site-specific cleavage of RNA.},
  author={M. A. Reynolds and T. A. Beck and P. Say and D. Schwartz and B. Dwyer and W. Daily and M. Vaghefi and M. D. Metzler and R. Klem and L. Arnold},
  journal={Nucleic acids research},
  year={1996},
  volume={24 4},
  pages={
          760-65
        }
}
  • M. A. Reynolds, T. A. Beck, +7 authors L. Arnold
  • Published 1996
  • Biology, Medicine
  • Nucleic acids research
  • We have designed and synthesized a series of novel antisense methylphosphonate oligonucleotide (MPO) cleaving agents that promote site-specific cleavage on a complementary RNA target. These MPOs contain a non- nucleotide-based linking moiety near the middle of the sequence in place of one of the nucleotide bases. The region surrounding the unpaired base on the RNA strand (i.e. the one directly opposite the non-nucleotide-linker) is sensitive to hydrolytic cleavage catalyzed by ethylenediamine… CONTINUE READING
    52 Citations

    Topics from this paper

    HYDROLYSIS OF BULGED NUCLEOTIDES IN HYBRIDS FORMED BY RNA AND IMIDAZOLE-DERIVATIZED OLIGO-2′-O-METHYLRIBONUCLEOTIDES
    • 4
    Sequence-specific artificial ribonucleases. I. Bis-imidazole-containing oligonucleotide conjugates prepared using precursor-based strategy.
    • 31
    • PDF
    The sequence-specific cleavage of RNA by artificial chemical ribonucleases.
    • R. Häner, J. Hall
    • Chemistry, Medicine
    • Antisense & nucleic acid drug development
    • 1997
    • 41
    Highly efficient catalytic RNA cleavage by the cooperative action of two Cu(II) complexes embodied within an antisense oligonucleotide.
    • 25
    • Highly Influenced
    • PDF

    References

    SHOWING 1-10 OF 37 REFERENCES
    Nonenzymatic hydrolysis of oligoribonucleotides.
    • R. Kierzek
    • Biology, Medicine
    • Nucleic acids research
    • 1992
    • 69
    Site-specific excision from RNA by RNase H and mixed-phosphate-backbone oligodeoxynucleotides.
    • 110
    • PDF
    Role of RNase H in hybrid-arrested translation by antisense oligonucleotides.
    • R. Walder, J. Walder
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1988
    • 365
    • PDF
    Hydrolysis of oligoribonucleotides: influence of sequence and length.
    • R. Kierzek
    • Biology, Medicine
    • Nucleic acids research
    • 1992
    • 53