Antisense inhibition of mitochondrial pyruvate dehydrogenase E1alpha subunit in anther tapetum causes male sterility.

@article{Yui2003AntisenseIO,
  title={Antisense inhibition of mitochondrial pyruvate dehydrogenase E1alpha subunit in anther tapetum causes male sterility.},
  author={Rika Yui and Satoru Iketani and Tetsuo Mikami and Tomohiko Kubo},
  journal={The Plant journal : for cell and molecular biology},
  year={2003},
  volume={34 1},
  pages={57-66}
}
We hypothesized that cytoplasmic male sterility (CMS) in sugar beet may be the consequence of mitochondrial dysfunctions affecting normal anther development. To test the hypothesis, we attempted to mimic the sugar beet CMS phenotype by inhibiting the expression of mitochondrial pyruvate dehydrogenase (PDH), which is essential for the operation of the tricarboxylic acid (TCA) cycle. Screening with a cDNA library of sugar beet flower buds allowed the identification of two PDH E1alpha subunit… CONTINUE READING

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