Antioxidant activity related to copper binding of native prion protein

  title={Antioxidant activity related to copper binding of native prion protein},
  author={D. Brown and C. Clive and S. J. Haswell},
  journal={Journal of Neurochemistry},
  • D. Brown, C. Clive, S. J. Haswell
  • Published 2001
  • Biology, Medicine
  • Journal of Neurochemistry
  • We have developed a method to affinity‐purify mouse prion protein (PrPc) from mouse brain and cultured cells. PrPc from mouse brain bound three copper atoms; PrPc from cultured cells bound between one and four copper atoms depending on the availability of copper in the culture medium. Purified PrPc exhibited antioxidant activity, as determined by spectrophotometric assay. Incubation of PrPc with the neurotoxic peptide, PrP106‐126, inactivated the superoxide dismutase‐like activity. Culture… CONTINUE READING
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