Antioxidant activity related to copper binding of native prion protein
@article{Brown2001AntioxidantAR, title={Antioxidant activity related to copper binding of native prion protein}, author={D. Brown and C. Clive and S. J. Haswell}, journal={Journal of Neurochemistry}, year={2001}, volume={76} }
We have developed a method to affinity‐purify mouse prion protein (PrPc) from mouse brain and cultured cells. PrPc from mouse brain bound three copper atoms; PrPc from cultured cells bound between one and four copper atoms depending on the availability of copper in the culture medium. Purified PrPc exhibited antioxidant activity, as determined by spectrophotometric assay. Incubation of PrPc with the neurotoxic peptide, PrP106‐126, inactivated the superoxide dismutase‐like activity. Culture… CONTINUE READING
Topics from this paper
211 Citations
Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells
- Biology, Medicine
- FEBS letters
- 2005
- 29
Prion protein fate governed by metal binding.
- Chemistry, Medicine
- Biochemical and biophysical research communications
- 2004
- 84
Aberrant metal binding by prion protein in human prion disease
- Biology, Medicine
- Journal of neurochemistry
- 2001
- 183
- PDF
Mapping the antigenicity of copper-treated cellular prion protein with the scrapie isoform
- Biology, Medicine
- Cellular and Molecular Life Sciences CMLS
- 2003
- 13
Metal imbalance and compromised antioxidant function are early changes in prion disease.
- Biology
- 2002
- 97
Metal imbalance and compromised antioxidant function are early changes in prion disease.
- Medicine
- The Biochemical journal
- 2002
- 103
- PDF
Role of the prion protein in copper turnover in astrocytes
- Biology, Medicine
- Neurobiology of Disease
- 2004
- 73
Oxidative impairment in scrapie‐infected mice is associated with brain metals perturbations and altered antioxidant activities
- Biology, Medicine
- Journal of neurochemistry
- 2001
- 151
Prion protein expression modulates neuronal copper content
- Biology, Medicine
- Journal of neurochemistry
- 2003
- 53
References
SHOWING 1-10 OF 39 REFERENCES
Normal prion protein has an activity like that of superoxide dismutase.
- Biology, Medicine
- The Biochemical journal
- 1999
- 522
- PDF
Prion protein expression aids cellular uptake and veratridine‐induced release of copper
- Biology, Medicine
- Journal of neuroscience research
- 1999
- 105
Selective oxidation of methionine residues in prion proteins.
- Biology, Medicine
- Biochemical and biophysical research communications
- 1999
- 79
Copper Stimulates Endocytosis of the Prion Protein*
- Biology, Medicine
- The Journal of Biological Chemistry
- 1998
- 585
- PDF
Prion protein selectively binds copper(II) ions.
- Medicine, Chemistry
- Biochemistry
- 1998
- 483
- Highly Influential
Prion Protein-Deficient Cells Show Altered Response to Oxidative Stress Due to Decreased SOD-1 Activity
- Biology, Medicine
- Experimental Neurology
- 1997
- 425
Prion protein expression and superoxide dismutase activity.
- Chemistry, Medicine
- The Biochemical journal
- 1998
- 262
- PDF
Differential release of cellular and scrapie prion proteins from cellular membranes by phosphatidylinositol-specific phospholipase C.
- Biology, Medicine
- Biochemistry
- 1990
- 242
Role of microglia and host prion protein in neurotoxicity of a prion protein fragment
- Biology, Medicine
- Nature
- 1996
- 501