• Corpus ID: 30657171

Antioxidant , Cytotoxic and Apoptotic Activities of Crude Extract of Alpinia purpurata on Cervical Cancer Cell Line T Research

@inproceedings{Anusooriya2016AntioxidantC,
  title={Antioxidant , Cytotoxic and Apoptotic Activities of Crude Extract of Alpinia purpurata on Cervical Cancer Cell Line T Research},
  author={Palanirajan Anusooriya and D. Malarvizhi and Chinthamony Arul Raj and Velliyur Kanniappan Gopalakrishnan},
  year={2016}
}

• P. Anusooriya, D. Malarvizhi, +1 author V. Gopalakrishnan
• Published 2016

Alpinia purpurata (Vieill.) K. Schum belongs in the Zingiberaceae family and is a very popular garden plant in India, it possesses moderate antibacterial and anticancer activities. To study the antioxidant, cytotoxic and apoptotic activities of n-hexane leaf extract of A. purpurata; antioxidant activity was determined by measuring (i) the scavenging effect of plant extract against 2, 2-diphenyl-1picryl hydrazyl (DPPH) and 2, 2′-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS), hydroxyl radical scavenging, superoxide radical scavenging, nitric oxide radical scavenging, hydrogen peroxide radical scavenging, metal chelating activity and (ii) reducing power capacity and ferric reducing power (FRAP). Cytotoxicity was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) and acridine orange/ethidium bromide staining to assess the anticancer activity of the crude extract on HeLa cells. The antioxidant activity of the plant extract was found to be close to that of standard ascorbic acid. DPPH radical scavenging activity IC50 value was 495 ± 1.43μg/ml and 440± 1.39μg/ml for extract and ascorbic acid respectively. ABTS radical scavenging activity IC50 value was 420± 2.26μg/ml and 400 ± 2.10μg/ml for extract and ascorbic acid respectively. The reducing power and ferric reducing activity of the extract increased with the increase of their concentrations. In MTT assay, leaf extract inhibited HeLa cells in a dose-dependent manner, showing cytotoxicity with IC50 of 41.25μg/ml. Morphological changes observed by fluorescent miscroscopy on the leaf extract treated cells stained with acridine orange/ethidium bromide suggested an apoptotic activity. This study clearly demonstrated that n-hexane leaf extract of Alpinia purpurata is a good antioxidant and induces apoptosis in HeLa cells.