Antimicrobial Susceptibility of Brucella melitensis Isolates in Peru

  title={Antimicrobial Susceptibility of Brucella melitensis Isolates in Peru},
  author={Ryan C. Maves and Rosa Castillo and Alfredo Guill{\'e}n and Benjamin J Espinosa and Rina A. Meza and Nereyda Espinoza and Gladys N{\'u}{\~n}ez and Luis Sánchez and Jes{\'u}s Chacaltana and David Cepeda and Sofía González and Eric R. Hall},
  journal={Antimicrobial Agents and Chemotherapy},
  pages={1279 - 1281}
ABSTRACT Brucellosis is an important public health problem in Peru. We evaluated 48 human Brucella melitensis biotype 1 strains from Peru between 2000 and 2006. MICs of isolates to doxycycline, azithromycin, gentamicin, rifampin, ciprofloxacin, and trimethoprim-sulfamethoxazole were determined by the Etest method. All isolates were sensitive to tested drugs during the periods of testing. Relapses did not appear to be related to drug resistance. 

Identification and In Vitro Antimicrobial Susceptibility of Brucella Species Isolated from Human Brucellosis

All isolates were noted to be sensitive to all the antimicrobial agents tested except for rifampicin where elevated MIC > 1 μg/mL was noted in 30 out of 41 isolates tested.

Characterization of Antibiotic Susceptibility of Brucella Spp Isolates with E-Test Method

It was shown that in-vitro trimethoprim-sulfamethoxsazole was the most effective antibiotic against B.melitensis, but enough care should be taken for the use of rifampicin which is frequently used for the treatment of brucellosis, an endemic disease in Turkey.

Antimicrobial susceptibility testing of Brucella melitensis isolated from patients with acute brucellosis in a centre of Iran

Although all the Brucella isolates were susceptible to rifampin, the antimicrobial susceptibility test should be recommended in patients with recurrent brucellosis or life-threatening organ involvement.

In vitro activity of Brucella melitensis isolates to various antimicrobials in Turkey

The in vitro activity of doxycycline and rifampicin, which are used in the classic treatment of brucellosis, was found to be very good and quinolones were found to have in vitroactivity against Brucella isolates.

In vitro antibiotic susceptibility testing of Brucella isolates from Egypt between 1999 and 2007 and evidence of probable rifampin resistance

This is the first report of high rates of probable resistance to rifampin among Brucella isolates from Egypt, and patients should be closely monitored while following standard treatment regimens.

Antimicrobial Susceptibility Pattern of Brucella Isolates from Abortion Cases in Animals in Northern India

The study revealed the existence of multiple drug resistance among strains of B. melitensis as well as B. abortus, and the antimicrobial sensitivity of 42 Brucella strains isolated from different animals to antibiotics and herbal antimicrobials was determined.

Antimicrobial Resistance in Leptospira, Brucella, and Other Rarely Investigated Veterinary and Zoonotic Pathogens.

Of the remaining veterinary fastidious pathogens, Lawsonia intracellularis is the most interesting from an antimicrobial resistance perspective because it can only be grown in cell culture, making in vitro susceptibility testing challenging.

Antibiogram Screening and Detection of Virulence-Associated Genes in Brucella Species Acquired from Cattle in South Africa’s Eastern Cape Province

Brucellosis is a widespread zoonotic illness, and it poses serious public health and economic risks. The purpose of this investigation is to look at the antimicrobial susceptibility of unpasteurized

Clinical and Diagnostic Aspects of Brucellosis and Antimicrobial Susceptibility of Brucella Isolates in Hamedan, Iran.

The aim of this study was to evaluate the antibiotic resistance pattern of Brucella melitensis clinical isolates by E-test method in Hamadan, west of Iran and recommended restricting the use of rifampin for treatment of brucellosis except as an alternative drug for special situations.

In vitro activities of antimicrobials against Brucella abortus isolates from cattle in Korea during 1998-2006.

In vitro activities of 13 antibiotics were assessed against 85 Brucella abortus isolates from naturally infected cattle in the Republic of Korea during 1998-2006, using broth microdilution test.



Actual antibiotic resistance pattern of Brucella melitensis in central Anatolia. An update from an endemic region.

All first line, and alternative antimicrobial agents could be used in various combinations in the treatment of human brucellosis and high MIC values of ceftriaxone and streptomycin are alarming, and should be closely monitored during the therapy.

Susceptibility of Mexican brucella isolates to moxifloxacin, ciprofloxacin and other antimicrobials used in the treatment of human brucellosis.

Fluoroquinolones, either first-generation or the newer 8-methoxi derivatives, might be useful in the therapy of brucellosis, which remains to be assessed in clinical trials.

MLVA genotyping of human Brucella isolates from Peru.

[Short communication: Investigation of in vitro antibiotic susceptibility of Brucella melitensis].

There was no serious resistance problem for TMP-SMX and doxycycline in this study, however, enough care should be taken for the use of rifampin which is frequently used for the treatment of tuberculosis and brucellosis which are endemic in this country.

Isolation of Brucella spp. from clinical specimens

For recovery of brucellae from clinical specimens, a lysis-concentration procedure was superior to the procedure of Castañeda (M. R. Castañeda, Proc. Soc. Exp. Biol. Med. 64:114-115, 1947).

Azithromycin and gentamicin therapy for the treatment of humans with brucellosis.

The results do not favor the use of azithromycin to treat brucellosis in humans.

Molecular Epidemiology of Brucella Genotypes in Patients at a Major Hospital in Central Peru

An annual variation in the diversity of genotypes was observed, possibly reflecting changes in sources of fresh dairy products, supply routes to city shops and markets, and the movement of infected dairy goat herds.

Quinolones for Treatment of Human Brucellosis: Critical Review of the Evidence from Microbiological and Clinical Studies

The rationale, as well as the outcomes, of quinolone use for brucellosis is examined and the most widely used regimens are the two aforementioned combinations that were recommended by the WHO.

Seroepidemiological and Microbiological Study of Brucellosis in Kuwait

Brucellosis is quite common in Kuwait and Kuwaiti and Bangladeshi nationals were most affected, and good in vitro activity against all isolates was found with tetracycline, gentamicin, amikacin, ciprofloxacin and streptomycin, and low activity with azithromycin and trimethoprim-sulfamethoxazole.

Comparison of culture techniques at different stages of brucellosis.

The lysis centrifugation technique is preferred for culturing Brucella spp. at all stages of brucellosis because it yields 25% more positive results and on average provides results 10 days earlier