Antimetabolite-induced apoptosis in Tenon's capsule fibroblasts.

  title={Antimetabolite-induced apoptosis in Tenon's capsule fibroblasts.},
  author={Jonathan Guy Crowston and Arne N. Akbar and Peter H Constable and Nicholas L Occleston and Julie T. Daniels and Peng T. Khaw},
  journal={Investigative ophthalmology & visual science},
  volume={39 2},
PURPOSE To determine whether treatment with mitomycin-c and 5-fluorouracil induces apoptotic death in cultured subconjunctival fibroblasts. METHODS Cultured human subconjunctival Tenon's capsule fibroblasts were exposed to 5-minute applications of mitomycin-C (up to 1 mg/ml) or 5-fluorouracil (up to 50 mg/ml) or phosphate-buffered saline solution (PBS). Fibroblast apoptosis was determined by cell morphology, apoptosis-specific protein expression, and DNA fragmentation by TdT-mediated dUTP… CONTINUE READING
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