Antilipolytic effect of prostaglandin E2 in perifused rat adipocytes.

  title={Antilipolytic effect of prostaglandin E2 in perifused rat adipocytes.},
  author={Bj\orn Richelsen and Steen B\onl\okke Pedersen},
  volume={121 4},
Previously, the antilipolytic effect of prostaglandin E2 (PGE2) has been investigated in conventional adipocyte incubations. To define the effect of PGE2 on lipolysis more clearly, isolated epididymal adipocytes were studied with the perifusion system. PGE2 inhibited isoproterenol (100 nM)- and theophylline (1 mM)-stimulated lipolysis in a concentration-dependent manner in both the perifusion system and conventional incubations. However, the half-maximally inhibitory concentration (ED50) of… CONTINUE READING