Antigenic differences between metastatic and nonmetastatic BSp73 rat tumor variants characterized by monoclonal antibodies.

@article{Matzku1989AntigenicDB,
  title={Antigenic differences between metastatic and nonmetastatic BSp73 rat tumor variants characterized by monoclonal antibodies.},
  author={Siegfried Matzku and Anke Wenzel and Shixi Liu and Margot Zoeller},
  journal={Cancer research},
  year={1989},
  volume={49 5},
  pages={1294-9}
}
Variants AS and ASML of the BSp73 rat tumor differ markedly with respect to morphology and to the capacity for spontaneous metastasis via the lymphatics. Monoclonal antibodies (MAbs) were raised in order to identify surface molecules associated with both phenotypes. Mice were immunized with either whole cells or isolated membranes and hybridoma supernatants were screened according to the criteria of selective binding to cultured cells of the variant used for immunization. Of two MAbs reacting… CONTINUE READING